avidin biotin complex kit (Vector Laboratories)
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Vector Laboratories
avidin biotin complex kit
Avidin Biotin Complex Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 12720 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/avidin+biotin+complex/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pmc13122667-89-14-17
Average 99 stars, based on 12720 article reviews
Avidin Biotin Complex Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 12720 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/avidin+biotin+complex/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pmc13122667-89-14-17
Average 99 stars, based on 12720 article reviews
avidin biotin complex kit - by Bioz Stars,
2026-09
99/100 stars
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Incubation:Article Title: Neuronal lipid droplets play a conserved and sex-biased role in maintaining whole-body energy homeostasis. Article Snippet: The following day, sections were washed with Tris-buffered saline (TBS; 50 mM, pH 7.4) and incubated for 90 min at RT with biotinylated donkey anti-chicken polyclonal secondary antibody (1:300 dilution; Jackson ImmunoResearch, 703-066-155; RRID: AB_2340355) in TBS containing 0.05% Triton X-100. .. Sections were washed in TBS and incubated for 1 h at RT in a 1:100 Article Title: Developmental trajectories of somatostatin-positive interneurons in the human auditory cortex from migration to maturation. Article Snippet: The sections were again rinsed thrice in PBS and incubated for 2 hours in a secondary antibody solution (Biotinylated anti-rat antibody raised in goat; 1:250 dilution, BA-9400, Vector Laboratories, Burlingame, CA, USA) at RT. .. After this step, sections were rinsed in PBS three times and incubated in a solution containing the Article Title: Microglia ablation is sufficient to prevent spontaneous withdrawal following perinatal morphine exposure. Article Snippet: Populations affected by the opioid epidemic include pregnant women and their offspring.. Infants exposed to opioids in utero are at risk of developing Neonatal Opioid Withdrawal Syndrome (NOWS), a combination of acute somatic withdrawal symptoms.. In adult rodents, there is evidence that opioid exposure and withdrawal alter microglia, but few studies have examined the effects of in utero exposure and neonatal withdrawal specifically on microglia. Article Title: Targeting of MARK2, but not other MARKs, suppresses TNBC progression by inhibition of the mutant p53-driven signaling pathway Article Snippet: Article history: Received 16 March 2025 Revised 7 June 2025 Accepted 9 June 2025 Available online 20 April 2026 Avidin-Biotin Assay:Article Title: Neuronal lipid droplets play a conserved and sex-biased role in maintaining whole-body energy homeostasis. Article Snippet: The following day, sections were washed with Tris-buffered saline (TBS; 50 mM, pH 7.4) and incubated for 90 min at RT with biotinylated donkey anti-chicken polyclonal secondary antibody (1:300 dilution; Jackson ImmunoResearch, 703-066-155; RRID: AB_2340355) in TBS containing 0.05% Triton X-100. .. Sections were washed in TBS and incubated for 1 h at RT in a 1:100 Article Title: Developmental trajectories of somatostatin-positive interneurons in the human auditory cortex from migration to maturation. Article Snippet: The sections were again rinsed thrice in PBS and incubated for 2 hours in a secondary antibody solution (Biotinylated anti-rat antibody raised in goat; 1:250 dilution, BA-9400, Vector Laboratories, Burlingame, CA, USA) at RT. .. After this step, sections were rinsed in PBS three times and incubated in a solution containing the Article Title: Microglia ablation is sufficient to prevent spontaneous withdrawal following perinatal morphine exposure. Article Snippet: Populations affected by the opioid epidemic include pregnant women and their offspring.. Infants exposed to opioids in utero are at risk of developing Neonatal Opioid Withdrawal Syndrome (NOWS), a combination of acute somatic withdrawal symptoms.. In adult rodents, there is evidence that opioid exposure and withdrawal alter microglia, but few studies have examined the effects of in utero exposure and neonatal withdrawal specifically on microglia. Article Title: Postnatal Development of Pyramidal Neurons Excitability and Synaptic Inputs in Mouse Gustatory Cortical Circuits Article Snippet: .. An Article Title: Alirocumab Attenuated Plaque Inflammation and PCSK9-Induced Proinflammatory Signalling in M1 Macrophages Independently of Lipid Lowering. Article Snippet: Non-specific protein binding was blocked with goat serum (1 h, room temperature), and primary anti-CD68 antibody (1:400) was incubated overnight at 4 ◦C, followed by a biotinylated anti-rabbit secondary antibody (1:500, 1 h, room temperature, Themo Fisher Scientific, Waltham, MA, USA, #11859200). .. The macrophage staining was developed by using the Article Title: Teneurin-2 and related proteins in reactive astrocytes after status epilepticus induction in adult rats Article Snippet: After that, the sections were incubated in the primary polyclonal antibody, anti-Ten-2 (1:100, Lot # K1910, sc-165674, Santa Cruz Biotechnology, CA, USA), or ADGRL1 (1:3000, Lot ALR021AN0102, ALR-021, Alomone Labs, Jerusalem, Israel), PBS-TX and 2% normal donkey serum for 48 h at 4 °C, followed by the specific biotinylated secondary antibody (1:800, lot # G0815, sc-2042, Santa Cruz Biotechnology or 1:500, lot #1515108, 711-065-152, Jackson Immunoresearch Laboratories Inc., PA, USA) for 90 min at 4 °C. .. Next, the sections were treated with Article Title: Targeting of MARK2, but not other MARKs, suppresses TNBC progression by inhibition of the mutant p53-driven signaling pathway Article Snippet: Article history: Received 16 March 2025 Revised 7 June 2025 Accepted 9 June 2025 Available online 20 April 2026 Article Title: Circuit-selective cognitive vulnerability to environmental stress: multi-domain assessment of space radiation in both sexes reveals countermeasure trade-offs Article Snippet: Following PBS washes, sections were incubated for 1 hour at room temperature with biotinylated secondary antibodies at 1:200 (donkey anti-goat IgG for DCX, Jackson ImmunoResearch, cat# 705-065-003; donkey anti-chicken IgG for GFP, Jackson ImmunoResearch, cat# 703-065-155; 1:200). .. After additional PBS washes, signal amplification was achieved using Amplification:Article Title: Microglia ablation is sufficient to prevent spontaneous withdrawal following perinatal morphine exposure. Article Snippet: Populations affected by the opioid epidemic include pregnant women and their offspring.. Infants exposed to opioids in utero are at risk of developing Neonatal Opioid Withdrawal Syndrome (NOWS), a combination of acute somatic withdrawal symptoms.. In adult rodents, there is evidence that opioid exposure and withdrawal alter microglia, but few studies have examined the effects of in utero exposure and neonatal withdrawal specifically on microglia. Article Title: Circuit-selective cognitive vulnerability to environmental stress: multi-domain assessment of space radiation in both sexes reveals countermeasure trade-offs Article Snippet: Following PBS washes, sections were incubated for 1 hour at room temperature with biotinylated secondary antibodies at 1:200 (donkey anti-goat IgG for DCX, Jackson ImmunoResearch, cat# 705-065-003; donkey anti-chicken IgG for GFP, Jackson ImmunoResearch, cat# 703-065-155; 1:200). .. After additional PBS washes, signal amplification was achieved using Staining:Article Title: Alirocumab Attenuated Plaque Inflammation and PCSK9-Induced Proinflammatory Signalling in M1 Macrophages Independently of Lipid Lowering. Article Snippet: Non-specific protein binding was blocked with goat serum (1 h, room temperature), and primary anti-CD68 antibody (1:400) was incubated overnight at 4 ◦C, followed by a biotinylated anti-rabbit secondary antibody (1:500, 1 h, room temperature, Themo Fisher Scientific, Waltham, MA, USA, #11859200). .. The macrophage staining was developed by using the Light Microscopy:Article Title: Teneurin-2 and related proteins in reactive astrocytes after status epilepticus induction in adult rats Article Snippet: After that, the sections were incubated in the primary polyclonal antibody, anti-Ten-2 (1:100, Lot # K1910, sc-165674, Santa Cruz Biotechnology, CA, USA), or ADGRL1 (1:3000, Lot ALR021AN0102, ALR-021, Alomone Labs, Jerusalem, Israel), PBS-TX and 2% normal donkey serum for 48 h at 4 °C, followed by the specific biotinylated secondary antibody (1:800, lot # G0815, sc-2042, Santa Cruz Biotechnology or 1:500, lot #1515108, 711-065-152, Jackson Immunoresearch Laboratories Inc., PA, USA) for 90 min at 4 °C. .. Next, the sections were treated with Control:Article Title: Teneurin-2 and related proteins in reactive astrocytes after status epilepticus induction in adult rats Article Snippet: After that, the sections were incubated in the primary polyclonal antibody, anti-Ten-2 (1:100, Lot # K1910, sc-165674, Santa Cruz Biotechnology, CA, USA), or ADGRL1 (1:3000, Lot ALR021AN0102, ALR-021, Alomone Labs, Jerusalem, Israel), PBS-TX and 2% normal donkey serum for 48 h at 4 °C, followed by the specific biotinylated secondary antibody (1:800, lot # G0815, sc-2042, Santa Cruz Biotechnology or 1:500, lot #1515108, 711-065-152, Jackson Immunoresearch Laboratories Inc., PA, USA) for 90 min at 4 °C. .. Next, the sections were treated with Conjugation Assay:Article Title: Circuit-selective cognitive vulnerability to environmental stress: multi-domain assessment of space radiation in both sexes reveals countermeasure trade-offs Article Snippet: Following PBS washes, sections were incubated for 1 hour at room temperature with biotinylated secondary antibodies at 1:200 (donkey anti-goat IgG for DCX, Jackson ImmunoResearch, cat# 705-065-003; donkey anti-chicken IgG for GFP, Jackson ImmunoResearch, cat# 703-065-155; 1:200). .. After additional PBS washes, signal amplification was achieved using |